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biochymickal_arts_2013 [2013-09-14 10:47] – [ongoing questions & open loops] maja | biochymickal_arts_2013 [2013-09-18 14:10] – nik | ||
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==== Biochymickal Arts 2013 ==== | ==== Biochymickal Arts 2013 ==== | ||
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+ | * in pictures https:// | ||
+ | * notes from each day | ||
+ | * [[biochymickal arts 20130913]] | ||
+ | * [[biochymickal arts 20130914]] | ||
+ | * [[biochymickal arts 20130915]] | ||
==== biochemistry / biology / bacteriology ==== | ==== biochemistry / biology / bacteriology ==== | ||
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* https:// | * https:// | ||
* growth medium | * growth medium | ||
- | * Potato dextrose agar (PDA) >> https:// | + | * Potato dextrose agar (PDA) [[PDA recipe|recipe]] |
- | * https:// | + | * Malt extract agar (MEA) [[MEA recipe|recipe]] |
+ | * others | ||
+ | * http:// | ||
==== biohacking ==== | ==== biohacking ==== | ||
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==== fermentation ==== | ==== fermentation ==== | ||
- | * SYN: ferment | + | * http:// |
- | * word fermentation is from the root of fervere "to boil, seethe" | + | * http://www.wildfermentation.com/ |
- | + | * http:// | |
- | + | | |
- | I (Maria) will make three samples: | + | * more info on kishk. http:// |
- | * A - salt-based fermentation (2%) | + | * La Paillasse: Paris Open Biotech Lab wiki with protocols http://www.lapaillasse.org/ |
- | * B - whey-based fermentation | + | |
- | * C - salt-based + fish | + | |
- | + | ||
- | A/B/C will come in different versions: | + | |
- | + | ||
- | | + | |
- | | + | |
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- | This is based on delivery on Wednesday 11 September afternoon. If you need them on Monday 9th, I can deliver in the morning, it just means that sample 3 will have to be opened after 2 days (so on Wednesday). | + | |
- | + | ||
- | There will be a total of 9 jars. For each, we said we would look at the vegetable tissue and liquid, so we are talking of about 18 or so slides. | + | |
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- | I will add to this: | + | |
- | + | ||
- | * D - sample of 2 weeks old (so inedible) sample of the fish kimchi you tried. | + | |
- | * E - sample from 2010 (unopened, we may find some mold on the top if we are lucky...). | + | |
- | * F - sample of kimchi juice that is 9 months old. | + | |
- | + | ||
- | ==== 20130913 ==== | + | |
- | * making PDA to prepare plates | + | |
- | * preparing plates, microwave pasteurisation (sub 120ºC sterilisation?) | + | |
- | * reading list and library subsection | + | |
- | * preparing cultures from 3x3 ferments (see above) | + | |
- | * cooking, eating, drinking (with various toasts) | + | |
- | + | ||
- | ==== 20130914 ==== | + | |
- | * maria' | + | |
====links and references==== | ====links and references==== | ||
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* " | * " | ||
- | fermentation | + | ==== Food ==== |
- | * http:// | + | |
- | * http:// | + | see [[biochymickal arts menu]] |
- | * http:// | + | |
- | * more info on funazushi (scroll down to posts on " | + | |
- | * more info on kishk. http:// | + | |
- | * La Paillasse: Paris Open Biotech Lab wiki with protocols http:// | + | |
- | + | ||
==== ongoing questions & open loops ==== | ==== ongoing questions & open loops ==== | ||
* how effective is a microwave for sterilisation? | * how effective is a microwave for sterilisation? | ||
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* GFP and/or Luciferase? | * GFP and/or Luciferase? | ||
* can we determine vitamin content of fermentations? | * can we determine vitamin content of fermentations? | ||
+ | * which Lactobacillus are able to produce vitamin C (see: L-ascorbate biosynthesis I, L-ascorbate biosynthesis II)? | ||
* which microorganisms can be chosen to increase vitamin production or production of specific amino acids | * which microorganisms can be chosen to increase vitamin production or production of specific amino acids | ||
* what sort of things can be done in a ' | * what sort of things can be done in a ' | ||
* good reference for korean techniques? | * good reference for korean techniques? | ||
+ | * "How can we reboot the ascorbate synthesis pathway in humans?" | ||
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