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biochymickal_arts_2013 [2013-09-14 10:51] – [ongoing questions & open loops] majabiochymickal_arts_2013 [2013-09-18 14:04] – nik
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 ==== Biochymickal Arts 2013 ==== ==== Biochymickal Arts 2013 ====
 +
 +  * in pictures https://secure.flickr.com/photos/foam/sets/72157635582680823/
 +  * notes from each day
 +    * [[biochymickal arts 20130913]]
 +    * [[biochymickal arts 20130914]]
 +    * [[biochymickal arts 20130915]]
  
 ==== biochemistry / biology / bacteriology ==== ==== biochemistry / biology / bacteriology ====
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     * https://en.wikipedia.org/wiki/Staining     * https://en.wikipedia.org/wiki/Staining
   * growth medium   * growth medium
-    * Potato dextrose agar (PDA) >> https://en.wikipedia.org/wiki/Potato_dextrose_agar or http://wiki.bugwood.org/Potato_dextrose_agar +    * Potato dextrose agar (PDA) [[PDA recipe|recipe]] 
-    * https://en.wikipedia.org/wiki/Growth_medium+    * Malt extract agar (MEA) [[MEA recipe|recipe]] 
 +    * others >> https://en.wikipedia.org/wiki/Growth_medium 
 +    * http://wiki.bugwood.org/Category:Fungal_Non-selective_Media
  
 ==== biohacking ==== ==== biohacking ====
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 ==== fermentation ==== ==== fermentation ====
    
-  * SYN: ferment - zymosis +  * see (other)
-  * word fermentation is from the root of fervere "to boil, seethe". +
- +
- +
-I (Maria) will make three samples: +
-  * A - salt-based fermentation (2%) +
-  * B - whey-based fermentation +
-  * C - salt-based + fish +
- +
-A/B/C will come in different versions: +
- +
-  - 6-days fermentation +
-  - 4-days fermentation +
-  - 2-days fermentation +
- +
-This is based on delivery on Wednesday 11 September afternoon. If you need them on Monday 9th, I can deliver in the morning, it just means that sample 3 will have to be opened after 2 days (so on Wednesday). +
- +
-There will be a total of 9 jars. For each, we said we would look at the vegetable tissue and liquid, so we are talking of about 18 or so slides. +
- +
-I will add to this: +
- +
-  * D - sample of 2 weeks old (so inedible) sample of the fish kimchi you tried. +
-  * E - sample from 2010 (unopened, we may find some mold on the top if we are lucky...). +
-  * F - sample of kimchi juice that is 9 months old. +
- +
-==== 20130913 ==== +
-  * making PDA to prepare plates +
-  * preparing plates, microwave pasteurisation (sub 120ºC sterilisation?) +
-  * reading list and library subsection +
-  * preparing cultures from 3x3 ferments (see above) +
-  * cooking, eating, drinking (with various toasts) +
- +
-==== 20130914 ==== +
-  * maria's introduction to fermentaion+
  
 ====links and references==== ====links and references====
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   * La Paillasse: Paris Open Biotech Lab wiki with protocols http://www.lapaillasse.org/wiki/index.php?title=Accueil   * La Paillasse: Paris Open Biotech Lab wiki with protocols http://www.lapaillasse.org/wiki/index.php?title=Accueil
    
 +
 +==== Food ====
 +
 +see [[biochymickal arts menu]]
 +
 ==== ongoing questions & open loops ==== ==== ongoing questions & open loops ====
   * how effective is a microwave for sterilisation? pressure cooker as autoclave?   * how effective is a microwave for sterilisation? pressure cooker as autoclave?
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   * GFP and/or Luciferase?   * GFP and/or Luciferase?
   * can we determine vitamin content of fermentations? what increases/inhibits vitamins?   * can we determine vitamin content of fermentations? what increases/inhibits vitamins?
 +  * which Lactobacillus are able to produce vitamin C (see: L-ascorbate biosynthesis I,  L-ascorbate biosynthesis II)? 
   * which microorganisms can be chosen to increase vitamin production or production of specific amino acids   * which microorganisms can be chosen to increase vitamin production or production of specific amino acids
   * what sort of things can be done in a 'kitchen lab' and what needs more specialised analytic equipment?    * what sort of things can be done in a 'kitchen lab' and what needs more specialised analytic equipment? 
   * good reference for korean techniques?   * good reference for korean techniques?
   * "How can we reboot the ascorbate synthesis pathway in humans?"   * "How can we reboot the ascorbate synthesis pathway in humans?"
 +
 +
  
  • biochymickal_arts_2013.txt
  • Last modified: 2021-12-06 12:10
  • by maja