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biochymickal_arts_2013 [2013-09-17 15:14] – nikbiochymickal_arts_2013 [2013-09-18 07:18] – nik
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     * https://en.wikipedia.org/wiki/Staining     * https://en.wikipedia.org/wiki/Staining
   * growth medium   * growth medium
-    * Potato dextrose agar (PDA) >> https://en.wikipedia.org/wiki/Potato_dextrose_agar or http://wiki.bugwood.org/Potato_dextrose_agar +    * Potato dextrose agar (PDA) [[PDA recipe|recipe]] 
-    * https://en.wikipedia.org/wiki/Growth_medium+    * Malt extract agar (MEA) [[MEA recipe|recipe]] 
 +    * others >> https://en.wikipedia.org/wiki/Growth_medium 
 +    * http://wiki.bugwood.org/Category:Fungal_Non-selective_Media
  
 ==== biohacking ==== ==== biohacking ====
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 ==== 20130913 ==== ==== 20130913 ====
-  * making [[PDA recipe|PDA]] to prepare plates+  * [[PDA recipe|making PDA]] to prepare plates
   * preparing plates, microwave pasteurisation (sub 120ºC sterilisation?)   * preparing plates, microwave pasteurisation (sub 120ºC sterilisation?)
   * reading list and library subsection   * reading list and library subsection
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   * La Paillasse: Paris Open Biotech Lab wiki with protocols http://www.lapaillasse.org/wiki/index.php?title=Accueil   * La Paillasse: Paris Open Biotech Lab wiki with protocols http://www.lapaillasse.org/wiki/index.php?title=Accueil
    
-==== ongoing questions & open loops ==== 
-  * how effective is a microwave for sterilisation? pressure cooker as autoclave? 
-  * good recipes for stains? 
-  * GFP and/or Luciferase? 
-  * can we determine vitamin content of fermentations? what increases/inhibits vitamins? 
-  * which Lactobacillus are able to produce vitamin C (see: L-ascorbate biosynthesis I,  L-ascorbate biosynthesis II)?  
-  * which microorganisms can be chosen to increase vitamin production or production of specific amino acids 
-  * what sort of things can be done in a 'kitchen lab' and what needs more specialised analytic equipment?  
-  * good reference for korean techniques? 
-  * "How can we reboot the ascorbate synthesis pathway in humans?" 
  
 ==== Food ==== ==== Food ====
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 The attempt to make “The pretty” jar The attempt to make “The pretty” jar
  
-Wild asparagus, chilly from the garden, cucumbers with the pattern incisions on the skin, slices of the beetroot , few cloves of garlic. All covert in salty brine ( 1/2 liter of water +-1tbsp of salt).+Wild asparagus, chilli from the garden, cucumbers with the pattern incisions on the skin, slices of the beetroot , few cloves of garlic. All covert in salty brine ( 1/2 liter of water +-1tbsp of salt). 
 After one day in brine i used all these ingredients for making sushi. The best combination was rice rolled in the fermented nasturtium leaves with the wild asparagus and a tiny slice of beetroot and garlic. After one day in brine i used all these ingredients for making sushi. The best combination was rice rolled in the fermented nasturtium leaves with the wild asparagus and a tiny slice of beetroot and garlic.
  
-The shiitake with dried nuts and raisins. +**Shiitake with dried nuts and raisins.**  
- +  * Soak nuts, shiitake and raisins in water over the night.  
-Soak nuts, shiitake and raisins in water over the night. +  * Squeeze out any liquid from the mushrooms but keep the juice a side. 
-Squeeze out any liquid from the mushrooms but keep the juice a side. +  * Put the nuts , shiitake and raisins in the jar. Mix the juice with salt. 
-Put the nuts , shiitake and raisins in the jar. Mix the juice with salt. +  * Pour it over the mushroom-nut mixture. Add a bit of tamari soya sauce.
-Pour it over the mushroom-nut mixture. Add a bit of tamari soya sauce.+
  
 Garlic fermented in soy sauce (from the book Japanese pickling recipes by Ikuko Hisamatsu) Garlic fermented in soy sauce (from the book Japanese pickling recipes by Ikuko Hisamatsu)
-10 whole garlic bulbs +  * 10 whole garlic bulbs 
-2 cups rice vinegar  +  * 2 cups rice vinegar  
-1 1/4 cups tamari soy sauce +  * 1 1/4 cups tamari soy sauce 
-2tbsp sugar+  * 2tbsp sugar 
 Peel the outer skin with your hands, but leave the whole bulb in tact, trim away the stems. Sterilize Peel the outer skin with your hands, but leave the whole bulb in tact, trim away the stems. Sterilize
 a small pickling jar, pack it with the garlic bulbs. Pour rice vinegar to cover let stand in a dark lace for two weeks. Discard 2/3 amount of vinegar ( you can use it for dressings).Mix soy sauce with sugar. Pour it over the garlic and cover it with the lid. You can eat it after one month.Just before serving cut horizontally in half. a small pickling jar, pack it with the garlic bulbs. Pour rice vinegar to cover let stand in a dark lace for two weeks. Discard 2/3 amount of vinegar ( you can use it for dressings).Mix soy sauce with sugar. Pour it over the garlic and cover it with the lid. You can eat it after one month.Just before serving cut horizontally in half.
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 I've made a variation of this recipe adding soaked shiitake mushrooms. Lets see in one month what will happen. I've made a variation of this recipe adding soaked shiitake mushrooms. Lets see in one month what will happen.
  
-Water kefir drinks +**Water kefir drinks** Put a spoon of dried rose petals with a few slices of ginger ( or the sweet basil seeds, or dried sage, or...) in a glass jar. Pour it over with the liquid of the 3 days old kefir culture, add 3cubes of sugar. Fill up the jar with filtered water. Kan be used in few hours or the next day. If you want to keep it for longer , put it into the fridge. If the drink becomes to strong divide between different jars and add water and sugar.
- +
-Put a spoon of dried rose petals with a few slices of ginger ( or the sweet basil seeds, or dried sage, or...) in a glass jar. +
-Pour it over with the liquid of the 3 days old kefir culture, add 3cubes of sugar. Fill up the jar with filtered water. Kan be used in few hours or the next day. If you want to keep it for longer , put it into the fridge. If the drink becomes to strong divide between different jars and add water and sugar.+
  
  
 +==== ongoing questions & open loops ====
 +  * how effective is a microwave for sterilisation? pressure cooker as autoclave?
 +  * good recipes for stains?
 +  * GFP and/or Luciferase?
 +  * can we determine vitamin content of fermentations? what increases/inhibits vitamins?
 +  * which Lactobacillus are able to produce vitamin C (see: L-ascorbate biosynthesis I,  L-ascorbate biosynthesis II)? 
 +  * which microorganisms can be chosen to increase vitamin production or production of specific amino acids
 +  * what sort of things can be done in a 'kitchen lab' and what needs more specialised analytic equipment? 
 +  * good reference for korean techniques?
 +  * "How can we reboot the ascorbate synthesis pathway in humans?"
  
  
    
  • biochymickal_arts_2013.txt
  • Last modified: 2021-12-06 12:10
  • by maja