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plant_roots_experiment [2011-09-27 10:19] lbillietplant_roots_experiment [2011-09-30 09:16] (current) lbilliet
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 ====Plant root experiment==== ====Plant root experiment====
-PARN project, Lionel+(part of the PARN project, Lionel)
  
-{{ :cad_drawing.png?nolink& |}}+===Abstract===
  
-{{ :parn_27sept_small03.jpg?nolink& |}}+The tips of the plant roots are highly sensitive areas, capable of detecting and interpreting a large range a environmental parameters such as water availability, gravity, nutrients concentration and mechanical forces. Those parameters determine the direction of the root's growth.
  
-{{ :parn_plant_roots_27sept_small02.jpg?nolink& |}}+Here, we decided to investigate the ability of a root to 'search for its way'. We designed a simple pattern of tiny channels (~ 1 mm² of section) to let a plant's root grow inside. At every branching, the root tip will have to make a choice, guided by its cellular sensory mechanims.
  
 +===Materials & methods===
  
 +{{:parn_microfluidics_small_bis.png?nolink}}\\
 +The idea is to carve 1mm deep grooves in a transparent layer. By adding another layer on it, the grooves becomes channels.
 +
 +{{:cad_drawing.png?nolink}}\\
 +A first, simple pattern for the channels (.xls drawing)
 +
 +{{:parn_plant_roots_27sept_small02.jpg?nolink}}\\
 +The pattern was grooved in a plexiglas plate by lasercut
 +
 +{{:parn_27sept_small03.jpg?nolink}}\\
 +Glass pipes connect the circuit with water stocks
 +
 +{{:parn_27sept_small01.jpg?nolink}}\\
 +A seed is inserted on the 'input' side of the circuit
 +
 +{{:parn_27_sept_scan_water_bis.jpg?nolink}}\\
 +First scan of the -- empty -- circuit
 +
 +{{:parn_img_1786_small.jpg?nolink}}\\
 +30 sept -- 11:15
  
 related: [[parn_project_lionel|PARN Project: Human-plant communication - research diary]] related: [[parn_project_lionel|PARN Project: Human-plant communication - research diary]]
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